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Sigma-Aldrich

MystiCq® microRNA cDNA Synthesis Mix

Sinônimo(s):

microRNA cDNA Synthesis Mix

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.55

técnica(s)

cDNA synthesis: suitable

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Descrição geral

Starting with total RNA or samples pre-enriched or microRNAs, the MystiCq® microRNA cDNA Synthesis Mix kit contains all the components necessary to convert mature microRNAs into cDNA templates for qPCR. Since microRNAs are not naturally polyadenylated, the first step in the cDNA synthesis process is to polyadenylate the microRNAs through a poly(A) polymerase reaction. Next, the ReadyScript reverse transcriptase, an oligo-dT adapter primer and other required reagents are added to convert the polyadenylated microRNAs into cDNA. The adapter primer includes a unique sequence at the 5′ end that is complementary to the sequence of the MystiCq Universal PCR Primer, another component of the MystiCq microRNA qPCR Assay System.The kit also contains a Positive Control Primer that is specific to SNORD44, a small nucleolar RNA that is widely expressed in most human tissues. Sufficient amounts of the Poly(A) Tailing Buffer and the MicroRNA cDNA Reaction mix are included in the kit to allow for the use of no poly(a) polymerase and no reverse transcriptase control reactions. To analyze individual microRNAs, use the MystiCq Universal PCR Primer (which targets the adapter primer sequence) together with the MystiCq microRNA qPCR Assay Primer and the MystiCq® microRNA SYBR® Green qPCR ReadyMix™.

Aplicação

MystiCq® microRNA cDNA Synthesis Mix has been used for first-strand cDNA synthesis by reverse-transcription of miRNAs for quantitative polymerase chain reaction (qPCR) starting from total RNA

Características e benefícios

  • Separate polyadenylation and reverse transcription steps allow for the possibility of optimization of protocol if necessary
  • cDNA produced may be archived for future use
  • Single RT reaction can provide up to 1000 qPCR reactions
  • Includes a positive control primer for use with most human tissues

Embalagem

Default reaction volume is 20 μL

Informações legais

MystiCq is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Somente componentes do kit

Nº do produto
Descrição
  • Poly (A) Tailing Buffer (5X)
  • Human Positive Control Primer
  • Nuclease-free Water
  • MystiCq® Universal PCR Primer
  • Poly (A) Polymerase
  • microRNA cDNA Reaction Mix
  • ReadyScript® Reverse Transcriptase

Código de classe de armazenamento

12 - Non Combustible Liquids

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