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SNC50

Sigma-Aldrich

mirPremier® microRNA Isolation Kit

1 sufficient for 50 preparations

Sinônimo(s):

microRNA Isolation Kit

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.52

Nível de qualidade

uso

sufficient for 50 preparations

técnica(s)

DNA extraction: suitable

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Descrição geral

Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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Aplicação

mirPremier® microRNA Isolation Kit has been used to:

  • extract total RNA containing miRNA from grape edible plant derived exosome-like nanoparticles (EPDEN).
  • isolate small RNA from S.sirkka and S. napiecek, and S. arctica.
  • extract miRNA, from frozen rat livers for miRNA analysis.

Características e benefícios

  • Designed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological sources.
  • Enables fast and efficient extraction and concentration of miRNA in 30 minutes for downstream applications.
  • Can extract high-purity miRNA with no detectable large RNA
  • No dangerous organic extractions are involved.

Informações legais

mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany

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Palavra indicadora

Warning

Classificações de perigo

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Dongxiao Su et al.
Food & function, 8(2), 808-815 (2017-01-26)
Dietary phenolics exhibit hypolipidemic activity by changing lipid metabolism-related microRNA (miRNA) expression. Quercetin 3-O-rutinoside-7-O-α-l-rhamnosidase (quercetin 3-rut-7-rha), rutin and (-)-epicatechin are the main phenolics in lychee (Litchi chinensis Sonn.) pulp. A previous study reported that quercetin 3-rut-7-rha and rutin had hypolipidemic
Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles.
Mu J
Molecular Nutrition And Food Research, 58(7), 1561-1573 (2014)
Jon Bråte et al.
Current biology : CB, 28(20), 3288-3295 (2018-10-16)
The emergence of multicellular animals was associated with an increase in phenotypic complexity and with the acquisition of spatial cell differentiation and embryonic development. Paradoxically, this phenotypic transition was not paralleled by major changes in the underlying developmental toolkit and
Abdulla Abdulla Sabana et al.
Planta, 251(4), 79-79 (2020-03-14)
Genome-wide analysis of small RNAs identifies somatic embryogenesis- specific miRNAs and their targets and provides novel insights into the mechanisms governing somatic embryogenesis in coconut, a highly in vitro recalcitrant species. Coconut, a major plantation crop of the tropics is

Artigos

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

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