MAK435

Acetylcholine Assay Kit

Sufficient for 100 colorimetric or fluorometric tests

• Código UNSPSC: 12161503
• NACRES: NA.84

Propriedades

• Nível de qualidade: 100
• entrada: cell culture(s); plasma; saliva; urine; milk; serum; tissue
• aplicação(ões): pharmaceutical
• método de detecção: colorimetric; fluorometric
• doença(s) relevante(s): cardiovascular diseases, Alzheimer′s disease; neurodegenerative disorders; neurological disorders
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Acetylcholine is a multifunctional neurochemical with diverse roles in the brain and various organ systems. It acts as a neurotransmitter, facilitating communication between neurons, myocytes, and cells in glandular tissues. The name "acetylcholine" originates from its chemical composition, which is an ester of acetic acid and choline. Tissues that utilize or respond to acetylcholine are termed cholinergic. While it functions as a neurotransmitter throughout the body, acetylcholine is particularly associated with the neuromuscular junction, where it triggers muscle activation by connecting motor neurons in the ventral spinal cord with muscles. Additionally, acetylcholine serves as a neurotransmitter in the autonomic nervous system, enabling communication between preganglionic and postganglionic neurons. It is the final product released by parasympathetic postganglionic neurons and plays vital roles in skeletal muscle movement, regulation of smooth and cardiac muscle, as well as in learning, memory, and mood.

Aplicação

The Acetylcholine Assay Kit may be used for:

• Neurological Research
• Drug Discovery
• Cell culture analysis

Características e benefícios

Convenient and Sensitive Measurement:

• Utilizes only 20 μL of the sample, providing an accurate determination of acetylcholine.
• Offers a wide detection range of 10-200 μM (colorimetric) and 0.4-10 μM (fluorometric), ensuring sensitivity across varying concentrations.

Simplified Process:

• Enjoy a hassle-free procedure requiring the addition of a single working reagent.
• Complete the assay with just a 30-minute reaction time, saving valuable time and effort.

Adequação

The kit is suitable for the quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism in serum, plasma, urine, saliva, milk, tissue, and cell culture etc.

Princípio

The Acetylcholine Assay Kit provides a simple, direct and high-throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase tobetaine and H₂O₂. The resulting H₂O₂ reacts with a specific dye to form a pink colored product. The color intensity at 570 nm or fluorescence intensity at λ_Ex = 530 nm/λ_Em = 585 nm is directly proportional to the acetylcholine concentration in the sample.

Outras notas

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Informações de segurança

• Frases de perigo: H412
• Declarações de precaução: P273 - P501
• Classificações de perigo: Aquatic Chronic 3
• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): 188.6 °F
• Ponto de fulgor (°C): 87 °C