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MAK223

Sigma-Aldrich

Aldolase Activity Colorimetric Assay Kit

Sufficient for 100 Colorimetric tests

Sinônimo(s):

Aldolase Colorimetric Assay Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

método de detecção

colorimetric

doença(s) relevante(s)

cancer; neurological disorders; hematological disorder

temperatura de armazenamento

−20°C

Informações sobre genes

human ... ALDOA(226) , ALDOB(229) , ALDOC(230)
mouse ... ALDOA(11674) , ALDOB(230163) , ALDOC(11676)
rat ... ALDOA(24189) , ALDOB(24190) , ALDOC(24191)

Descrição geral

Aldolase (fructose-bisphosphate aldolase; EC 4.1.2.13) catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three isoforms of aldolase (A, B, and C) are differentially expressed during development. Aldolases have been recognized as positive regulators of the Wnt signaling pathway. Deficiency of aldolases is associated with myopathy and hemolytic anemia.

The Aldolase Activity Colorimetric Assay Kit is a simple and high throughput assay for measuring aldolase activity in serum, plasma, and a variety of tissues and cells. Aldolase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of aldolase is the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.2 at 37 °C.
Aldolase (fructose-bisphosphate aldolase; EC 4.1.2.13) catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three isoforms of aldolase (A, B, and C) are differentially expressed during development. Aldolases have been recognized as positive regulators of the Wnt signaling pathway. Deficiency of aldolases is associated with myopathy and hemolytic anemia

Características e benefícios

Compatible with high-throughput handling systems.

Adequação

Suitable for the detection of Aldolase activity in biological samples such as tissue, cells, serum, and plasma.

Princípio

The Aldolase Activity Colorimetric Assay Kit is a simple and high throughput assay for measuring aldolase activity in serum, plasma, and a variety of tissues and cells. Aldolase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of aldolase is the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.2 at 37 °C.

Somente componentes do kit

Nº do produto
Descrição
  • Aldolase Assay Buffer
  • Aldolase Substrate
  • Aldolase Enzyme Mix
  • Aldolase Developer
  • NADH Standard
  • Aldolase Positive Control

Pictogramas

Corrosion
GHS05

Palavra indicadora

Danger

Frases de perigo

H314,H412

Classificações de perigo

Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials

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Aldolase positively regulates of the canonical Wnt signaling pathway.
Caspi M, et al.
Molecular Cancer, 13(1), 164-164 (2014)
Human aldolase A natural mutants: relationship between flexibility of the C-terminal region and enzyme function.
Esposito G, et al.
The Biochemical Journal, 380(1), 51-56 (2004)
Unraveling the structural and functional features of an aldolase A mutant involved in the hemolytic anemia and severe rhabdomyolysis reported in a child.
Esposito G, et al.
Blood, 105(2), 905-906 (2005)
Zhenchang Guo et al.
Molecular & cellular proteomics : MCP, 20, 100053-100053 (2021-02-10)
Esophageal squamous cell carcinoma (ESCC) is an aggressive malignancy with poor therapeutic outcomes. However, the alterations in proteins and posttranslational modifications (PTMs) leading to the pathogenesis of ESCC remain unclear. Here, we provide the comprehensive characterization of the proteome, phosphorylome
Biochemical markers of muscular damage.
Brancaccio P, et al
Clinical Chemistry and Laboratory Medicine, 48(6), 757-767 (2010)
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