Pular para o conteúdo
Merck
Todas as fotos(2)

Documentos Principais

Visualizar Toda a Documentação

MAK020

Sigma-Aldrich

Fructose-6-Phosphate Assay Kit

sufficient for 100 fluorometric tests

Sinônimo(s):

Fructose-6-P Quantification Kit

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(2)

About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 fluorometric tests

método de detecção

fluorometric

doença(s) relevante(s)

cancer

temperatura de armazenamento

−20°C

Descrição geral

Fructose-6-phosphate (F6P) is a glycolytic pathway intermediate produced by the isomerization of glucose-6-phosphate by phosphoglucose isomerase. F6P is further phosphorylated to fructose 1,6-biphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. F6P can also be shunted from glycolysis to the non-oxidative branch of the pentose phosphate pathway as a means of generative pentose phosphates for nucleotide synthesis. F6P levels are elevated in rapidly proliferating cells such as cancer cells. The Fructose-6-phosphate Assay Kit is a highly sensitive and simple fluorescence-based method of quantifying F6P in a variety of samples.

Aplicação

Fructose-6-Phosphate Assay Kit has been used to measure fructose-6-phosphate (F-6-P) and glucose-6-phosphate (G6P).

Adequação

Suitable for quantifying Fructose-6-Phosphate in a variety of samples

Princípio

Fructose-6-phosphate concentration is determined by a coupled enzyme reaction, which results in a fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the fructose-6-phosphate present. Typical sensitivities for this kit are 0.01 to 0.5 nmoles of F6P.

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

188.6 °F - closed cup

Ponto de fulgor (°C)

87 °C - closed cup

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
7E28K06890
6M28K06890
5E07K06890
5E073K0689
5B13K06890

Não está vendo a versão correta?

Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.

Pesquise por COA

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Reciprocal regulation between O-GlcNAcylation and tribbles pseudokinase 2 (TRIB2) maintains transformative phenotypes in liver cancer cells.
Yao B, et al.
Cellular Signalling, 28(11) (2016)
Proteomic and biochemical basis for enhanced growth yield of Enterobacter sp. LCR1 on insoluble phosphate medium.
Kumar A and Rai L C
Microbiological research, 170 (2015)
Lauriane Galle-Treger et al.
The Journal of allergy and clinical immunology, 145(2), 502-517 (2019-11-19)
Allergic asthma is a chronic inflammatory disorder characterized by airway hyperreactivity (AHR) and driven by TH2 cytokine production. Group 2 innate lymphoid cells (ILC2s) secrete high amounts of TH2 cytokines and contribute to the development of AHR. Autophagy is a
Mingliang Wang et al.
Biochemical and biophysical research communications, 504(2), 400-405 (2018-06-13)
This study aims to demonstrate the role of miR-182 in the glucose metabolism of NSCLC cells and the potential mechanism. MTT Cytotoxicity Assay was used to measure the function of differentially expressed miR-182 on two NSCLC cell lines proliferation. Metabolite
Sicheng Gao et al.
DNA and cell biology, 38(4), 286-296 (2019-02-15)
Abnormal expression of O-Linked β-N-acetylglucosamine (O-GlcNAc) and β-catenin is a general feature of cancer and contributes to transformed phenotypes. In this study, we identified the interaction between O-GlcNAc and β-catenin, and explored their effects on the progression of liver cancer.
Exibir todos os documentos relacionados

Artigos

Aerobic Glycolysis and the Warburg Effect

We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica