M7927

Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Número MDL: MFCD01633615
• Código UNSPSC: 12352203
• NACRES: NA.44

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: IgG fraction of antiserum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen, ERK-1 44 kDa; antigen, ERK-2 42 kDa
• reatividade de espécies: mouse, rat, human
• técnica(s): immunoprecipitation (IP): 5 μg using NIH 3T3 mouse fibroblast lysates; microarray: suitable; western blot: 1:5,000 using NIH 3T3 mouse fibroblast lysate or rat brain extract
• nº de adesão UniProt: P27361
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... MAPK3(5595) ; mouse ... Mapk3(26417) ; rat ... Mapk3(50689)

Descrição

Descrição geral

Mitogen-activated protein kinase 3 (ERK1) is mapped to human chromosome 16p11.2. It has a serine/threonine domain and a transactivation domain (TAD) and a nuclear localization sequence (NLS).

Especificidade

By immunoblotting, the antibody reacts with ERK-1 and ERK-2.

Imunogênio

synthetic peptide corresponding to amino acids 351-368 of human ERK-1.

Aplicação

Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit has been used in:

• immunofluorescence
• immunoprecipitation
• western blotting.

Ações bioquímicas/fisiológicas

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), c-Jun N-terminal kinases (JNK) and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK1 (p44) and ERK2 (p42) require the dual phosphorylation in the catalytic kinase domain by MEKs for their full activity. ERK1 is phosphorylated on Tyr204 and Thr202, and ERK2 on Tyr187 and Thr185. ERK1 and 2 may also undergo autophosphorylation on these residues. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.

forma física

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)