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Merck
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Key Documents

M7408

Millipore

MacConkey Agar

NutriSelect® Basic, suitable for microbiology

Sinônimo(s):

Enteric bacteria selective medium, MacConkey′s medium

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About This Item

Número CE:
Número MDL:
Código UNSPSC:
41171606
eCl@ss:
42040102
NACRES:
NA.74

esterilidade

non-sterile

Nível de qualidade

forma

powder

embalagem

bottle of 250 g
bottle of 500 g

fabricante/nome comercial

NutriSelect® Basic

cor

light yellow

aplicação(ões)

agriculture
environmental
food and beverages
pharmaceutical
water monitoring

microbiology

adequação

Citrobacter spp.
Escherichia coli
Gardnerella spp.
coliforms

Descrição geral

MacConkey Agar is used for the isolation, identification, and enumeration of lactose fermenting and lactose non-fermenting enteric bacteria.The media is accepted by the Standard Methods for the Examination of milk and dairy Products and pharmaceutical preparations. Peptone in the media provides nitrogenous and carbonaceous compounds, long-chain amino acids, vitamins, and other essential growth factors. Lactose is the fermentable sugar that causes acid production and a color change of the indicator when the pH of the medium falls below 6.8. Sodium chloride maintains the osmotic equilibrium. The selective action of this medium is attributed to crystal violet and bile salts, which are inhibitory to most species of gram-positive bacteria. Gram-negative bacteria usually grow well on the medium and are differentiated by their ability to ferment lactose. Lactose fermenting strains form red or pink colonies and are surrounded by a zone of acid-precipitated bile. Lactose non-fermenting strains, such as Shigella and Salmonella, form colorless, transparent colonies and typically do not alter the appearance of the medium.

Aplicação

MacConkey Agar is a differential plating medium recommended for the detection and isolation of coliforms and intestinal pathogens from stool, urine, water, and other material.

Ações bioquímicas/fisiológicas

MacConkey Agar is considered as a selective and differential media. It is useful in differentiating between the lactose fermenting and non-lactose fermenting bacteria. Thus, it is useful in the isolation of gram negative bacteria. Primary plating medium is used to differentiate members of the coliform group.

Características e benefícios

NutriSelect® Basic are powdered culture media offering
  • Quality control with basic growth promotion test
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

Nota de preparo

Suspend 50 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Pour into sterile petri plates. Dry the surface of the gel before inoculation.

Nota de análise

Microbiologically tested.

Nota de rodapé

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informações legais

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


Certificados de análise (COA)

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