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L7651

Sigma-Aldrich

Lisozima

lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein, for molecular biology

Sinônimo(s):

Mucopeptídeo N-acetilmuramoil-hidrolase, Muramidase

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About This Item

Número CAS:
12650-88-3
Número da licença da enzima:
3.2.1.17 (BRENDA, IUBMB)
Número CE:
235-747-3
Número MDL:
MFCD00131557
Código UNSPSC:
12352204

grau

for molecular biology

forma

lyophilized powder

atividade específica

≥40,000 units/mg protein

peso molecular

single-chain 14.3 kDa

composição

protein, ≥90%

nº de adesão UniProt

P00698

temperatura de armazenamento

−20°C

Informações sobre genes

chicken ... LYZ(396218)

Procurando produtos similares? Visita Guia de comparação de produtos

Aplicação

A enzima quebra as paredes celulares das bactérias; usada para preparar esferoplastos.
Lysozyme hydrolyzes the β-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine, a polysaccharide backbone of peptidoglycans in the cell wall structure of many microorganisms. This is particularly useful for lysing Gram-positive and Gram-negative bacteria for subsequent nucleic acid extraction.

Ações bioquímicas/fisiológicas

A lisoenzima hidrolisa as ligações β(1→4) entre o ácido N-acetilmurâmico e os resíduos de N-acetil-D-glicosamina no peptidoglicano e entre resíduos de N-acetil-D-glicosamina na chitodextrina. Células gram-positivas são bastante susceptíveis a essa hidrólise, pois suas paredes celulares têm uma alta proporção de peptidoglicano. Bactérias gram-negativas são menos susceptíveis devido à presença de uma membrana externa e uma menor proporção de peptidoglicano. Contudo, essas células podem ser hidrolisadas na presença de EDTA que quela íons metálicos na membrana bacteriana externa.

A enzima fica ativa em um amplo intervalo de pH (6,0 a 9,0). Em pH 6,2, atividade máxima é observada ao longo de um intervalo de forças iônicas (0,02 a 0,100 M) que em pH 9,2 (0,01 a 0,06 M).

Características e benefícios

  • Highly purified by repeated crystallization and dialysis
  • Each lot is use-tested for isolation of plasmid DNA from E. coli

Definição da unidade

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

forma física

Lyophilized powder, essentially salt-free

Nota de preparo

3× crystallized

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How can I reconstitute Lysozyme from chicken egg white, Product L7651?

    For E. coli cell lysis, use a freshly prepared lysozyme solution (10 mg/mL) in 10 mM Tris-HCl, pH 8.0. The product is also soluble in water (10 mg/mL) yielding a clear to slightly hazy colorless solution.

  4. How stable are solutions of Lysozyme from chicken egg white, Product L7651?

    Aqueous solutions should retain activity for at least one month when stored between 2-8 °C.

  5. Is Lysozyme from chicken egg white, Product L7651, suitable for bacterial lysis prior to DNA purification?

    Each lot is verified to be suitable for use as a lysing agent in purifying plasmid DNA from E. coli. You can also use this product in combination with the GenElute™ Bacterial Genomic DNA Kit (Product No. NA2100) to purify genomic DNA from Gram-positive bacteria.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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