I2521

ITS+1 Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

• Sinônimo(s): Insulin-transferrin-sodium selenite, linoleic-BSA supplement
• Código UNSPSC: 12352205
• NACRES: NA.75

Propriedades

• esterilidade: sterile-filtered
• Nível de qualidade: 500
• linha de produto: BioReagent
• Formulário: liquid
• técnica(s): cell culture | mammalian: suitable
• Impurezas: endotoxin, tested
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Serum-free culture environment avoids the variability and contaminants that can be introduced by serum. Serum-free formulations that substitute a purified form of the factors normally supplied by serum are suitable for many in vitro growth and differentiation studies. These factors include insulin, transferrin, selenium, pyruvate, and ethanolamine. The addition of other components varies greatly, depending on the cell type being studied and the basal medium employed. ITS is a mixture of insulin, human transferrin (partially iron-saturated), and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g., MEM, RPMI-1640) and complex media (e.g., Ham′s F-12, DME/F-12, MEM) with sodium pyruvate.

Aplicação

ITS+1 Liquid Media Supplement (100×) has been used as a supplement in high-glucose Dulbecco′s Modified Eagle Medium (DMEM) for differentiation of mouse mesenchymal stem cells (mMSCs) to chondrocytes. It has also been used as a supplement in basal differentiation medium for differentiation of human-induced pluripotent stem cell (hiPSC) into osteoblasts under serum-free conditions.

Insulin-transferrin-sodium selenite, linoleic-BSA has been used for serum-free cell culture applications.ITS+1 Liquid Media Supplement (100×) has been used in culturing cell lines and for the preparation of immortalisation medium for the production of cell lines from ′Immortomouse′ embryos.

Componentes

Contains 1.0 mg/ml bovine insulin, 0.55 mg/ml human transferrin (substantially iron-free), 0.5 μg/ml sodium selenite, 50 mg/ml bovine serum albumin and 470 μg/ml linoleic acid at the 100x concentration.

Nota de preparo

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type ABEK (EN14387) respirator filter