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H5505

Sigma-Aldrich

Histone from calf thymus

Type III-S

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About This Item

Número CAS:
9064-47-5
Beilstein:
1895465
Número MDL:
MFCD00131329
Código UNSPSC:
41106305
NACRES:
NA.51

fonte biológica

bovine (calf) thymus

tipo

Type III-S

Formulário

powder

concentração

≥60% protein (biuret)

técnica(s)

activity assay: suitable

solubilidade

H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow

temperatura de armazenamento

2-8°C

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Aplicação

Histone is suitable for use:
  • as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 protein
  • in Cdk5 activity assay
  • in histone H1 kinase assay
  • as a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activity
  • as an acceptor of 32P from [γ-32P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells

Ações bioquímicas/fisiológicas

Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain.

Características e benefícios

Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.

Outras notas

Characterized as mainly subgroup f1 by SDS-PAGE

Nota de preparo

Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962).

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
SLCL0914
SLCK9015
SLBF2552V
051M73502V
051M73502

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