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GE28-9893-31

Sigma-Aldrich

HiLoad® 16/600 Superdex® 30 pg

Cytiva 28-9893-31, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

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About This Item

Código UNSPSC:
41115700
NACRES:
SB.52

Materiais

bed support (polyamide)
glass column
polypropylene fiber (reinforced glass)

peso molecular

<10000 Da (globular proteins)

embalagem

pkg of 1 ea

fabricante/nome comercial

Cytiva 28-9893-31

Parâmetros

<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)

volume do leito

120 mL

coluna C × D.I.

60 cm × 16 mm

Matriz

agarose dextran composite
 dextran-agarose particle platform
polymeric

Grupo ativo da matriz

dextran-agarose phase
dextran-agarose phase

Tamanho médio da partícula

34 μm

cleaning

1-14

Faixa de trabalho

3-12

técnica de separação

size exclusion (SEC)

Categorias relacionadas

Descrição geral

HiLoad® 16/600 Superdex® 30 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography separating peptides.

Aplicação

Superdex® prep grade (pg) is produced by covalent bonding of Dextran to highly cross-linked Agarose.The separation properties of these media are determined by the Dextran component. Steep selectivity curves give excellent resolving power for peptides and Proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Características e benefícios

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to ÄKTA® design systems.

Adequação

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography separating peptides

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Informações legais

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictogramas

Flame
GHS02

Palavra indicadora

Warning

Frases de perigo

H226

Código de classe de armazenamento

3 - Flammable liquids

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Certificados de análise (COA)

Lot/Batch Number

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Artigos

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Protocolos

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

Conteúdo relacionado

Separation Options with Superdex®

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

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