GE28-9909-45

Superdex^® 200 Increase 5/150 GL

Cytiva 28-9909-45, column L × I.D. 15 cm × 5 mm, 8.6 μm particle size

• Sinônimo(s): Size-exclusion chromatography column
• Código UNSPSC: 41115700
• NACRES: SB.52

Propriedades

• Materiais: glass column; polyethylene filter
• linha de produto: Superdex^®
• peso molecular: 10000-600000 Da (globular proteins)
• fabricante/nome comercial: Cytiva 28-9909-45
• embalagem: 1 ea of
• Parâmetros: <0.75 mL/min flow rate; <0.75 ml/min flow rate; <3.0 Mpa (725 psi); 4-50 μL sample volume
• volume do leito: ~3 mL
• coluna C × D.I.: 15 cm × 5 mm
• Matriz: agarose dextran composite; polymeric
• Grupo ativo da matriz: phenyl phase
• tamanho de partícula: 8.6 μm
• cleaning: 1-14
• Faixa de trabalho: 3-12
• técnica de separação: size exclusion (SEC)

Descrição

Descrição geral

Superdex™ 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~ 10 000 to ~ 600 000.

Superdex™ 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex™ Increase columns deliver higher resolution purification in shorter run times.

Superdex™ 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations. The outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions.

Superdex™ 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose™ 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate.

This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.

Características e benefícios

• Enhanced performance: improved resolution and runtime compared to predecessor Superdex™ 200.
• Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity.
• High flow rates: rigid beads give excellent pressure/flow properties.

Adequação

Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride

Well suited for rapid purity checks and quick screenings of experimental conditions.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

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Informações legais

Superdex is a registered trademark of Cytiva

Informações de segurança

• Pictogramas: GHS02
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de classe de armazenamento: 3 - Flammable liquids