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Documentos Principais

GE17-3712-22

HIS Mag Sepharose Excel

Cytiva 17-3712-22, pack of 10 × 1 mL

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

IMAC immobilized with Ni

embalagem

pack of 10 × 1 mL

fabricante/nome comercial

Cytiva 17-3712-22

Parâmetros

Ambient temp. range

Matriz

highly cross-linked spherical agarose including magnetite

Descrição geral

His Mag Sepharose Excel are magnetic beads designed for rapid, small-scale purification and screening of his-tagged proteins consisting of an Immobilized Metal Affinity Chromatography (IMAC) resin precharged with nickel ions that are strongly bound to a chelating ligand.

Small-scale capture, purification, and screening of histidine-tagged proteins that are expressed and secreted into supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells in HyClone HyCell CHO cell culture media.

The base of the matrix is based on Sepharose resin, incorporated with magnetite. His Mag Sepharose is available as 10% slurry in three different pack sizes: 2 x 1 mL, 5 x 1 mL, and 10 x 1mL.

Our ISO 13485 accredited manufacturing center of excellence adheres to the standards in the manufacturing process, ensuring the highest quality and compliance with international medical device regulations.

Características e benefícios

  • Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture
  • Purify histidine-tagged proteins directly from samples that contain metal ion-stripping agents and save time and effort
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • Screen histidine-tagged proteins in parallel with high reproducibility and reliable results
  • Capture histidine-tagged protein from different sample volumes; from low to high milliliter scale
  • Simplify handling using visible and dense Sepharose-based magnetic beads

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% ethanol, 5% medium slurry)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Sepharose is a trademark of Cytiva

Pictogramas

Environment

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


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Artigos

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

Protocolos

This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from GE Healthcare.

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from GE Healthcare.

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