G0774
Glycerokinase from Bacillus stearothermophilus
buffered aqueous solution, ≥75 units/mg protein (biuret)
Sinônimo(s):
ATP:glycerol 3-phosphotransferase, Glycerol Kinase
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About This Item
Produtos recomendados
fonte biológica
Bacillus sp. (Bacillus steaarothermophilus)
Nível de qualidade
Ensaio
≥5.0 mg protein/mL (biuret)
Formulário
buffered aqueous solution
atividade específica
≥75 units/mg protein (biuret)
condição de armazenamento
dry at room temperature
cor
beige
aplicação(ões)
life science and biopharma
temperatura de armazenamento
2-8°C
Informações sobre genes
Bacillus sp. ... glpK(89613566)
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Descrição geral
Research area: Cell Signaling
Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.
Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.
Aplicação
Glycerokinase from Bacillus stearothermophilus has been used in steady-state kinetics studies.
Ações bioquímicas/fisiológicas
Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.Oxidized glycerol-3-phosphate results in dihydroxyacetone phosphate which enters either glycolysis or gluconeogenesis. Elevated glycerol is observed in glycerol kinase deficiency leading to pseudohypertriglyceridemia.
Definição da unidade
One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.
forma física
Stabilized solution in Tris buffer, pH 7.3
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Purification and properties of glycerol kinase from Escherichia coli.
S I Hayashi et al.
The Journal of biological chemistry, 242(5), 1030-1035 (1967-03-10)
N Zwaig et al.
Journal of bacteriology, 102(3), 753-759 (1970-06-01)
The activity of glycerol kinase is rate-limiting in the metabolism of glycerol by cells of Escherichia coli. A mutant strain producing a glycerol kinase resistant to inhibition by fructose-1,6-diphosphate grows faster than its wild-type parent on glycerol as the sole
Ioanna Gouni-Berthold et al.
The British journal of nutrition, 107(11), 1694-1706 (2011-10-15)
Animal and human studies suggest that a malleable protein matrix (MPM) from whey decreases plasma lipid concentrations and may positively influence other components of the metabolic syndrome such as glucose metabolism and blood pressure (BP). The primary objective of this
Daria V Cherkashina et al.
Rejuvenation research, 14(6), 661-667 (2011-10-20)
Abstract Effects of stem and progenitor cells or their compounds on recipient cells are investigated intensively today. In spite of this, their ability to interact with native cells and the final targets affected by them, particularly biochemical parameters that characterize
Laurence Portois et al.
International journal of molecular medicine, 29(6), 1121-1126 (2012-03-20)
Second generation n3-PUFA-depleted rats represent a good animal model of metabolic syndrome as they display several features of the disease such as liver steatosis, visceral obesity and insulin resistance. The goal of our study was to investigate the influence of
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