F9015
Fluorescamine
≥98% (TLC), powder, used for detection of primary amines
Sinônimo(s):
4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione
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About This Item
Fórmula empírica (Notação de Hill):
C17H10O4
Número CAS:
Peso molecular:
278.26
Beilstein:
921143
Número CE:
Número MDL:
Código UNSPSC:
12352204
ID de substância PubChem:
NACRES:
NA.32
Produtos recomendados
Ensaio
≥98% (TLC)
forma
powder
cor
off-white to yellow
pf
153-157 °C (lit.)
solubilidade
acetone: 50 mg/mL
temperatura de armazenamento
room temp
cadeia de caracteres SMILES
O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14
InChI
1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
chave InChI
ZFKJVJIDPQDDFY-UHFFFAOYSA-N
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Descrição geral
Fluorescamine, a heterocyclic dione, reacts with primary amines, amino acids, peptides, and protein to form a fluorescent product. Excess reagent and its degradation products are non-fluorescent. The fluorescence of a solution containing protein plus fluorescamine is equivalent to the quantity of free amine groups present. This is the basic principle of a fluorescent protein assay. Fluorescamine is used as a derivatizing agent for the identification of various compounds used in the pharmaceutical industry, such as oseltamavir, penicillamine, sulfonamide residues, lisinopril, methotrexate, vigabatrin, and gabapentin.
Aplicação
Fluorescamine has been used as a stain to localize granular proteins on the surface of foxtail millet starch as well as protein in foxtail millet flour through confocal scanning laser microscopy (CSLM). It has also been used for photometric determination of para-aminobenzoic acid.
Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
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S Udenfriend et al.
Science (New York, N.Y.), 178(4063), 871-872 (1972-11-24)
Fluorescamine is a new reagent for the detection of primary amines in the picomole range. Its reaction with amines is almost instantaneous at room temperature in aqueous media. The products are highly fluorescent, whereas the reagent and its degradation products
S Udenfriend et al.
Science (New York, N.Y.), 178(4063), 871-872 (1972-11-24)
Fluorescamine is a new reagent for the detection of primary amines in the picomole range. Its reaction with amines is almost instantaneous at room temperature in aqueous media. The products are highly fluorescent, whereas the reagent and its degradation products
Hai-Teng Li et al.
Food chemistry, 330, 127328-127328 (2020-06-23)
Granular protein is an important structural feature in determining starch digestibility. High-amylose wheat starch (HAWS) with >80% amylose content contains more granular protein than wild-type starch. As analyzed by mass spectrometry-based proteomics, granular-bound starch synthase (GBSS) is the major granular
P C Hollman et al.
The American journal of clinical nutrition, 62(6), 1276-1282 (1995-12-01)
Quercetin is a dietary antioxidant that prevents oxidation of low-density lipoproteins in vitro. Intake of quercetin was inversely associated with coronary heart disease mortality in elderly Dutch men. However, the extent of absorption of quercetin in humans is unclear. The
Wen-Hsien Tsai et al.
Journal of chromatography. A, 1217(49), 7812-7815 (2010-11-04)
A simple sugaring-out assisted liquid-liquid extraction method combined with high-performance liquid-chromatography with fluorescence detection (HPLC-FL) was developed for the extraction and determination of sulfonamides in honey. Sample preparation consisted of acid hydrolysis to release sugar-bound sulfonamides. After derivatization with fluorescamine
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