E2520

Anti-Epidermal Growth Factor antibody, Mouse monoclonal

clone EGF-10, purified from hybridoma cell culture

• Sinônimo(s): Anti-CEGF
• Número MDL: MFCD00239669
• Código UNSPSC: 51111800
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• conjugado: unconjugated
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: EGF-10, monoclonal
• forma: buffered aqueous solution
• peso molecular: antigen 6 kDa
• reatividade de espécies: human
• técnica(s): dot blot: 0.1 μg/mL using recombinant human EGF; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human tissue; indirect ELISA: suitable; microarray: suitable; neutralization: suitable; radioimmunoassay: suitable; western blot: suitable
• Isotipo: IgG1
• nº de adesão UniProt: P01133
• aplicação(ões): research pathology
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... EGF(1950)

Descrição

Descrição geral

Monoclonal Anti-Epidermal Growth Factor (EGF) (mouse IgG1 isotype) is derived from the EGF-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Epidermal growth factor (EGF) is a single chain polypeptide hormone (6 kDa). EGF protein exists largely in a β-sheet conformation, with a C-terminal hairpin that may be involved in receptor binding. Human EGF is found in body fluids such as urine, milk, saliva, sweat, and seminal fluid and has its highest concentration in α-granules of blood platelets.

Especificidade

Specifically reacts with both native and recombinant human EGF. The antibody does not cross-react with mouse EGF.

Imunogênio

recombinant human EGF

Aplicação

Monoclonal Anti-Epidermal Growth Factor antibody has been used in dot-blot, radioimmunoassay, enzyme linked immunosorbent assay (ELISA), immunohistology, immunoblotting, immunostaining.

Monoclonal Anti-Epidermal Growth Factor antibody produced in mouse was used to study rat immune cells by flow cytometry to detect the presence or absence of hormone and hormone-like molecules in response to EGF treatment.

Ações bioquímicas/fisiológicas

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.

Epidermal growth factor (EGF) is a potent growth-promoting factor for a variety of tissue cells in vivo and in vitro. EGF binding induces phosphatidylinositide hydrolysis and generation of diacylglycerol (DAG). Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis and synthesis of DNA, RNA and proteins. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells. The biological role of EGF includes the inhibition of gastric acid secretion, support of growth and differentiation during fetal development, neuromodulation in the central nervous system and stimulation of epidermal growth and keratinization.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves