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Merck
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Key Documents

DUO92020

Sigma-Aldrich

Duolink® In Situ PLA® Probe Anti-Human PLUS

Affinity purified Donkey anti-Human IgG (H+L)

Sinônimo(s):

in situ Proximity Ligation Assay, Protein Protein Interaction Kit

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About This Item

Código UNSPSC:
41105331
NACRES:
NA.41

fonte biológica

donkey (Polyclonal)

forma do anticorpo

affinity purified immunoglobulin (Secondary antibody)

linha de produto

Duolink®

reatividade de espécies

human

técnica(s)

immunofluorescence: suitable
proximity ligation assay: suitable

adequação

suitable for brightfield
suitable for fluorescence

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Aplicação

Duolink® proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting and more.

To perform a complete Duolink® PLA® in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.
Specificity

PLA probe anti-Human reacts with whole molecule human IgG and the light chains of other human immunoglobulin?s. The PLA Probe Anti-Human Plus has minimal cross-reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, mouse, rabbit, rat, and sheep serum proteins. A MINUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.

Application Note

Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

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Características e benefícios

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Componentes

This product is comprised of the following:
  • 5x PLA Probe Anti-Human PLUS - Donkey anti-human secondary antibody conjugated to oligonucleotide PLUS
  • 1x Blocking Solution - Reagent for blocking of the sample
  • 1x Antibody Diluent - For dilution of PLA probes and primary antibodies

See datasheet for more information.

Informações legais

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

Exclamation markEnvironment

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Aquatic Chronic 2 - Skin Sens. 1

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3


Certificados de análise (COA)

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Protocolos

Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.

Conteúdo relacionado

Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay

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