DUO96010
Duolink® PLA Multicolor Probemaker Kit - Red
Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events
Sinônimo(s):
in situ Proximity Ligation Assay Kit, Protein Protein Interaction Kit
About This Item
Produtos recomendados
linha de produto
Duolink®
técnica(s)
immunofluorescence: suitable
multiplexing: suitable
proximity ligation assay: suitable
fluorescência
λex 594 nm; λem 624 nm (Texas Red®)
λex 594 nm; λem 624 nm (red)
adequação
suitable for fluorescence
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Aplicação
Red fluorescence detection reagents are often used with Texas Red® filter.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells are required.
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Características e benefícios
- Visualize and detect multiple proteins events in one experiment.
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Detection of low abundant proteins due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Faster and more simple than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Components
Duolink® PLA Multicolor Probemaker Kit - Red contains all the necessary reagents to perform the conjugations of Red Oligo A and Red Oligo B directly to primary antibodies. The resulting PLA probes (Red A and Red B) must be used together and cannot be paired with any other Duolink
This product is comprised of the following:
- Red Oligo A DUO86010A
- Red Oligo B DUO86010B
- Conjugation Buffer DUO82033
- Stop Reagent DUO82034
- Storage Solution DUO82035
See datasheet for more information.
To perform a Multicolor Duolink® PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink® PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink® Wash Buffers and Mounting Medium.
Informações legais
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Aquatic Chronic 2 - Skin Sens. 1
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Artigos
Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.
Protocolos
Duolink® PLA Multicolor Detection Protocol
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