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DMN70

Sigma-Aldrich

GenElute™ Direct mRNA Miniprep Kits

sufficient for 70 purifications

Sinônimo(s):

GenElute™ Direct mRNA Miniprep Kit, GenElute™ mRNA Kit, Gen Elute

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About This Item

Código UNSPSC:
41105501
NACRES:
NA.52

uso

sufficient for 70 purifications

Nível de qualidade

200

temperatura de armazenamento

15-25°C

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Descrição geral

The GenElute™ Direct mRNA Miniprep kit provides a convenient format to isolate polyadenylated mRNA directly from mammalian cells and tissues. The direct mRNA isolation procedure is based on that of Badley. Up to 107 mammalian cells or 40 mg tissue are lysed and homogenized, either with the filtration columns provided or with
a mechanical homogenizer. RNase is eliminated during a 10 minute proteinase K digestion. Sodium chloride is added, and polyadenylated RNA is captured on oligo(dT) polystyrene beads during a 10 minute incubation. For further enrichment, RNA may be released from the beads into fresh lysis solution and recaptured with the original beads. After 3 washes in a spin column, purified mRNA is eluted in 100 μl of 10 mM Tris-HCl, pH 7.4.

Aplicação

GenElute™ Direct mRNA Miniprep Kit has been used to isolate mRNA from various tissues and cells.
The GenElute Direct Kit mRNA kit provides convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues.

Características e benefícios

  • mRNA captured on oligo(dT) polystyrene beads in 10 minutes, with no mixing or rocking (Fig. 1)
  • Poly (A)+ mRNA isolated from total RNA in 40 minutes (Fig. 2) or 60 minutes directly from cells and tissues (Fig. 3)
  • Oligo(dT) polystyrene beads require fewer wash steps

Outras notas

Use for isolating mRNA directly from mammalian cells or tissues.
For additional information, please see www.sigma-aldrich.com/mrna.

Informações legais

GenElute is a trademark of Sigma-Aldrich Co. LLC

Somente componentes do kit

Nº do produto
Descrição
  • Collection tube 70 ea
  • Elution solution 10 mL
  • Filtration columns with tubes 70 ea
  • Lysis solution 120 mL
  • 5 M NaCl 8 mL
  • Oligo(dT)-polystyrene beads 2 mL
  • Proteinase K 25 mg
  • Spin columns with tubes 70 ea
  • 40% Glycerol solution 3 mL
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Palavra indicadora

Danger

Frases de perigo

H290,H315,H319,H334,H335

Classificações de perigo

Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

Identification and charactrization of glucoamylase from the fungus Thermomyces lanuginosus
Thor S Thorsen
Biochimica et Biophysica Acta (2006)
Human embryonic stem cell differentiation on tissue engineering scaffolds: effects of NGF and retinoic acid induction.
Inanc B
Tissue Engineering: Part A, 14(6), 955-964 (2008)

Artigos

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

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