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D6063

Sigma-Aldrich

REDTaq® SuperPak DNA Polymerase

Taq for routine PCR with inert dye; with 10X buffer & dNTP mix

Sinônimo(s):

DNA polymerase with dNTP, DNA polymerase with loading dye, Taq DNA polymerase

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About This Item

Código UNSPSC:
41106300
NACRES:
NA.55

Nível de qualidade

Formulário

liquid

uso

sufficient for 250 reactions

Características

dNTPs included
hotstart: no

concentração

1 unit/μL

técnica(s)

PCR: suitable

cor

red

entrada

purified DNA

adequação

suitable for PCR

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Descrição geral

REDTaq® SuperPak DNA Polymerase is Sigma′s Taq DNA Polymerase mixed with an inert red dye. REDTaq® SuperPak DNA Polymerase is a convenient package, that includes all the necessary components for a PCR reaction except primers, DNA template and water. It consists of Sigma′s high quality REDTaq DNA polymerase, 10 mM ultrapure deoxynucleotide mix and 10´ PCR reaction buffer. The dye provides quick recognition of reactions to which enzyme has been added as well as visual confirmation of complete mixing.

Aplicação

REDTaq® SuperPak DNA Polymerase has been used in:
  • reverse transcriptase-polymerase chain reaction (RT-PCR)
  • nested PCR
  • routine PCR amplification

Características e benefícios

  • Contains inert dye that enables visual confirmation of enzyme addition and direct loading onto agarose gel without the need for an additional loading dye
  • Suitable for nested PCR
  • Contains ultrapure 10mM dNTP mix in a separate vial

Informações legais

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
SuperPak is a trademark of Sigma-Aldrich Co. LLC

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS

  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 includedSDS

  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology ReagentSDS

produto relacionado

Nº do produto
Descrição
Preços

Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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A Jóźwik et al.
Veterinary research communications, 29(4), 347-359 (2005-03-09)
Two pairs of primers were prepared, both localized within the sequences of the nucleoprotein gene (NP) of canine distemper virus (CDV). A number of experiments were done to optimize the conditions of RT-PCR and nested PCR methods. The nucleic acids
M Yeste-Velasco et al.
Neuroscience, 159(3), 1135-1147 (2009-04-10)
Increasing evidence implicates the c-Jun NH(2)-terminal kinase (JNK) pathway in the regulation of apoptosis in neurodegenerative diseases. In this study, we examined the neuroprotective effect of SP600125, a selective JNK inhibitor, in cerebellar granule cells (CGNs) deprived of serum and
M Yeste-Velasco et al.
Neuropharmacology, 53(2), 295-307 (2007-07-07)
Recent studies have demonstrated that neuronal reentry in the cell cycle and specifically the expression of the transcription factor E2F-1, constitutes a pathway that may be involved in neuronal apoptosis after serum and potassium withdrawal. Other enzymes such as glycogen
Vishnu Chintalgattu et al.
Journal of molecular and cellular cardiology, 35(3), 277-286 (2003-04-05)
Vascular endothelial growth factor (VEGF), produced predominantly by endothelial cells, is involved in angiogenesis and mitogenesis. Myofibroblasts (myoFb) are phenotypically transformed fibroblast-like cells found at the site of myocardial infarction. Since myoFb play a role in tissue repair/remodeling at the
D Alvira et al.
Neuroscience, 147(3), 746-756 (2007-06-23)
The mechanism involved in neuronal apoptosis is largely unknown. Studies performed on neuronal cell cultures provide information about the pathways which orchestrate the process of neuronal loss and potential drugs for the treatment of neurological disorders. In the present study

Protocolos

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Method for PCR using genomic DNA or other complex templates

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