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Documentos Principais

CN402-05

Sigma-Aldrich

Canine Chondrocytes: CnC (Cryovial)

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About This Item

Código UNSPSC:
41106514
NACRES:
NA.81

fonte biológica

canine articular cartilage (normal)

embalagem

pkg of 500,000 cells

fabricante/nome comercial

Cell Applications, Inc

modo de crescimento

Adherent

cariótipo

2n = 78

morfologia

chondrocyte

técnica(s)

cell culture | mammalian: suitable

doença(s) relevante(s)

arthritis

Condições de expedição

dry ice

temperatura de armazenamento

−196°C

Descrição geral

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Canine Chondrocytes (CnC) are derived from normal canine articular cartilage where they produce and maintain the extracellular matrix of cartilage. Following monolayer culturing, CnC can be grown in alginate microspheres to study chondrocyte proliferation and metabolism in a three-dimensional in vitro system. Alginate microspheres provide more physiological conditions for the chondrocytes and allow them to sustain normal proteoglycans production and retention. Because of the prevalence of degenerative joint disease in geriatric dogs, CnC can be a useful tool for veterinary practice.

Canine Chondrocytes (CnC) were utilized to evaluate anti-inflammatory and anti-oxidant activity of Glyco-Flex III (Yáñez, 2008) and trans-pterostilbene (Remsberg, 2008). They were also used in material studies aimed to develop optimal surfaces/scaffolds for engineered tissues, cellular arrays, biosensors and lab-on-chip devices (Wright, 2012; Shaik 2013a,b).

Origem de linhagem celular

Cartilage

Aplicação

production and maintenance of extracellular matrix, cartilage, collagen, differentiation and de-differentiation, signal transduction, apoptosis, differentiation, drug screening, gene expression, cytokine production, agarose assays, chondrocyte adhesion to medical implants, scaffolds for cartilage regeneration

Componentes

Canine Chondryocyte Basal Medium containing 10% FBS & 10% DMSO

Nota de preparo

  • 1st passage, >500,000 cells in Canine Chondryocyte Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 10 doublings

Rotina de subcultura

Please refer to the CnC Culture Protocol.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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