CLL1223

Safe Harbor Landing Pad Cell Line THP-1 Monocytes

human male peripheral blood (Source Disease: Acute monocytic leukemia)

• Código UNSPSC: 41106514
• NACRES: NB.01

Propriedades

• Nome do produto: Safe Harbor Landing Pad Cell Line THP-1 Monocytes,
• fonte biológica: human male peripheral blood (Source Disease: Acute monocytic leukemia)
• Nível de qualidade: 200
• Formulário: frozen liquid (Vial of Frozen Cells)
• modo de crescimento: Suspension
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição geral

The STR profile of this cell line matches that of its parental cell line ATCC^®Catalog No. TIB-202. THP-1 are a human monocyte cell line isolated from the peripheral blood of a male infant with acute monocytic leukemia. The cells are phagocytic and lack surface and cytoplasmic immunoglobulins. Differentiation can be induced with TPA.

Descrição de linhagem celular

These cells were derived from the peripheral blood of a 1-year old male with acute monocytic leukemia. These cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. They stain positive for α-napthhyl butyrate esterase, produce lysozymes, and are phagocytic (both latex beads and sensitized erythrocytes). They can restore the response of purified T lymphocytes to Concanavalin A, show increased CO2 production on phagocytosis, and can be differentiated into macrophage-like cells using, for example, DMSO.

Aplicação

This product is a human THP-1 monocyte cell line in which a landing pad cassette has been integrated into the AAVS1 safe harbor locus using CompoZr^® Zinc Finger Nuclease technology. The mKATE2 fluorescence gene was integrated following the EF1a promoter and flanked by unique Cre-lox sites. The design of this landing pad cassette allows for easy, fast, and affordable genetic modification using Cre recombinase. mKATE2 can easily be exchanged for a payload of the user′s choice using Cre recombinase and a targeting vector with appropriate lox sites. Cells can then be sorted via fluorescence-activated cell sorting (FACS) for loss of mKATE2 expression as a surrogate for successful integration of the targeting vector. Approximately 7-10 days are required for loss of the mKATE2 signal in successfully targeted cells. See technical bulletin for detailed protocols.

Características e benefícios

These cells contain the mKATE2 fluorescence gene flanked by unique Cre-lox sites inserted in the AAVS1 safe harbor gene. These THP-1 cells are grown in suspension with a doubling time of approximately 24 hours.

Qualidade

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Informações legais

ATCC is a registered trademark of American Type Culture Collection

CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS05
• Palavra indicadora: Warning
• Frases de perigo: H290
• Declarações de precaução: P234 - P390
• Classificações de perigo: Met. Corr. 1
• Código de classe de armazenamento: 8A - Combustible corrosive hazardous materials
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable