CLL1221

Safe Harbor Landing Pad Cell Line Jurkat T-Lymphocytes

human male peripheral blood (Source Disease: Acute T cell leukemia)

• Sinônimo(s): T-Lymphocyte Cell Line
• Código UNSPSC: 41106514
• NACRES: NA.81

Propriedades

• product name: Safe Harbor Landing Pad Cell Line Jurkat T-Lymphocytes,
• fonte biológica: human male peripheral blood (Source Disease: Acute T cell leukemia)
• Nível de qualidade: 200
• forma: frozen liquid (Vial of Frozen Cells)
• modo de crescimento: Suspension
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição geral

The STR profile of this cell line matches that of its parental cell line ATCC^® Catalog No. TIB-152. Jurkat T lymphocytes are a human, acute T cell lymphoma cell line isolated in the late 1970s from the peripheral blood of a young male patient suffering from T cell leukemia. The cells possess a pseudodiploid karyotype and have been characterized as expressing CD3 and, upon stimulation, interleukin-2.

Descrição de linhagem celular

These cells are a human, acute T cell lymphoma cell line isolated from the peripheral blood of a young male suffering from T cell leukemia. The cells possess a pseudodiploid karyotype, express CD3 and, upon stimulation, IL-2.

Aplicação

This product is a human Jurkat T-lymphocyte cell line in which a landing pad cassette has been integrated into the AAVS1 safe harbor locus using CompoZr^® Zinc Finger Nuclease technology. The mKATE2 fluorescence gene was integrated following the EF1a promoter and flanked by unique Cre-lox sites. The design of this landing pad cassette allows for easy, fast, and affordable genetic modification using Cre recombinase. mKATE2 can easily be exchanged for a payload of the user′s choice using Cre recombinase and a targeting vector with appropriate lox sites. Cells can then be sorted via fluorescence-activated cell sorting (FACS) for loss of mKATE2 expression as a surrogate for successful integration of the targeting vector. Approximately 7-10 days are required for loss of the mKATE2 signal in successfully targeted cells. See technical bulletin for detailed protocols.

Características e benefícios

These cells contain the mKATE2 fluorescence gene flanked by unique Cre-lox sites inserted in the AAVS1 safe harbor gene. These Jurkat cells are grown in suspension with a doubling time of approximately 22 hours.

Qualidade

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Meio de cultura

RPMI modified to contain 2mM L-glutamine, 10mM HEPES, 1mM sodium pyruvate, and 1500mg/L sodium bicarbonate. Add 10% FBS(Catalog Number F2442).

Informações legais

ATCC is a registered trademark of American Type Culture Collection

CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS05
• Palavra indicadora: Warning
• Frases de perigo: H290
• Declarações de precaução: P234 - P390
• Classificações de perigo: Met. Corr. 1
• Código de classe de armazenamento: 8A - Combustible corrosive hazardous materials
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable