CHA00011
EUR-2019-nCoV Assays for detection of SARS-CoV-2 RNA
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About This Item
Código UNSPSC:
41105500
NACRES:
NA.51
Produtos recomendados
Formulário
solution (in 10 mM Tris, 1 mM EDTA)
embalagem
pkg of 2 vials
faixa de pH
7.5-8.5
Condições de expedição
ambient
temperatura de armazenamento
−20°C
Descrição geral
EUR-2019-nCoV Assays are for qPCR (5′ nuclease assay) detection of SARS-CoV-2.
The product is primers and probes, the sequences of which were designed and made publicly available by Charité Institute of Virology – Universitätsmedizin Berlin.
For additional details, please visit SigmaAldrich.com/ncov.
The product is primers and probes, the sequences of which were designed and made publicly available by Charité Institute of Virology – Universitätsmedizin Berlin.
For additional details, please visit SigmaAldrich.com/ncov.
Características e benefícios
The forward and reverse primers in each tube come with the following features:
The Dual-Labeled Probe in each tube comes with the following features:
- DNA bases
- No modifications
- Desalt purified
- 8 nmol each E_Sarbeco & 10 nmol each Modified RdRp
The Dual-Labeled Probe in each tube comes with the following features:
- DNA bases
- 6-FAM™ reporter
- BXQ (Block All Quencher—SigmaAldrich.com/bxq)
- HPLC purified
- 4 nmol E_Sarbeco & 5 nmol Modified RdRp
Componentes
The product includes 2 individual tubes of 20X primer and probe pools for quantification of SARS-CoV-2 genes. Assays included are E_Sarbeco and Modified RdRp (formerly Discrimatory) genes.
The forward primer, reverse primer, and probe are at final concentrations of 8, 8, and 4 μM (E_Sarbeco); and, 10, 10, and 5 μM (Modified RdRp), respectively.
E_Sarbeco Assay:
Forward Primer ACAGGTACGTTAATAGTTAATAGCGT
Reverse Primer ATATTGCAGCAGTACGCACACA
Dual-Labeled Probe ACACTAGCCATCCTTACTGCGCTTCG
Modified RdRp Assay:
Forward Primer AAATGGTCATGTGTGGCGGT
Reverse Primer GTTAAAAACACTATTAGCATAAGCAGTTGT
Dual-Labeled Probe CAGGTGGAACCTCATCAGGAGATGC
The forward primer, reverse primer, and probe are at final concentrations of 8, 8, and 4 μM (E_Sarbeco); and, 10, 10, and 5 μM (Modified RdRp), respectively.
E_Sarbeco Assay:
Forward Primer ACAGGTACGTTAATAGTTAATAGCGT
Reverse Primer ATATTGCAGCAGTACGCACACA
Dual-Labeled Probe ACACTAGCCATCCTTACTGCGCTTCG
Modified RdRp Assay:
Forward Primer AAATGGTCATGTGTGGCGGT
Reverse Primer GTTAAAAACACTATTAGCATAAGCAGTTGT
Dual-Labeled Probe CAGGTGGAACCTCATCAGGAGATGC
Informações legais
FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
Código de classe de armazenamento
12 - Non Combustible Liquids
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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