Ri-1

96090512, human blood, Lymphoblastoid

• Sinônimo(s): Ri-1 Cell Line
• Código UNSPSC: 41106514

Propriedades

• Nome do produto: Ri-1, 96090512
• fonte biológica: human blood
• modo de crescimento: Suspension
• cariótipo: 92XXXX/69XXX,t(1,2)(p32,q34)t(2,8)(p11,q24)t(14,18
• morfologia: Lymphoblastoid
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian B-cell lymphoma

Descrição de linhagem celular

The cell line Ri-1 has been established from the peripheral blood of a 48-year-old female patient with B-cell lymphoma. This EBV-negative cell line with 14q+ abnormality was shown to be at an intermediate stage of B-cell differentiation. Ri-1 expresses surface and cytoplasmic immunoglobulin (IgM kappa), HLA-DR, CD20, CD38 and FMC7. Rearranged heavy-light chain genes and one rearranged kappa light-chain gene was detected as well as a kappa and lambda light-chain gene in germ-line configuration.

Aplicação

Study of B-cell lymphoma-leukaemia

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cell growth is very slow after resuscitation; large amounts of debris present.

Outras notas

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