OV56

96020759, human ovary, Epithelial

• Sinônimo(s): OV-56 Cells
• Código UNSPSC: 41106514

Propriedades

• product name: OV56, 96020759
• fonte biológica: human ovary
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: VEGF
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human ovarian serous carcinoma (ascites) stage IV, poorly differentiated

Descrição de linhagem celular

The OV56 cell line was established from ascitic fluid derived from a 46-year-old female patient diagnosed with ovarian cancer. The tumour was characterised as stage IV, poorly differentiated. Immunocytochemical staining of the cells was reported to show strong staining for cytokeratin (CK), weak staining for carcinoembryonic antigen (CEA) and strong staining for human milk fat globulin (HMFG-2). Production of vascular endothelial growth factor (VEGF) has been detected by ELISA. The patient had received sequential treatment with cisplatin and cyclophosphamide, cisplatin and etoposide and subsequently tamoxifen.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8,9
D16S539: 10,13
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 9,11
vWA: 15,19

Meio de cultura

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) + 0.5 ug/ml hydrocortisone + 10ug/ml insulin

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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