M15

95102517, mouse kidney, Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: M15, 95102517
• fonte biológica: mouse kidney
• modo de crescimento: Adherent
• cariótipo: Hyperdiploid
• morfologia: Epithelial
• produtos: WT1 (expressed gene)
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse mesonephric epithelium, polyoma virus large T transformed

Descrição de linhagem celular

The WT1-expressing mesonephric cell line M15 (alias Meso15) was established from mouse mesonephros transgenically expressing the large T protein of polyoma virus under the control of the early viral enhancer. As a tumour suppresser gene with a key role in urogenital development, WT1 is implicated as predisposition gene in the pathogenesis of Wilms′ tumour (WT).

Aplicação

Gene expression and protein studies connected to kidney development and Wilms′ tumourigenesis.

Meio de cultura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. NB: Cell growth is impaired at lower densities.

Outras notas

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