TGP 55

95062833, mouse pancreas, Polymorph

• Código UNSPSC: 41106514

Propriedades

• product name: TGP 55, 95062833
• fonte biológica: mouse pancreas
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Polymorph
• produtos: Somatostatin and Insulin
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse pancreatic small cell anaplastic carcinoma

Descrição de linhagem celular

TGP 55 has been obtained from a small cell anaplastic carcinoma arising in the pancreas of a transgenic Tg(Ela-1SV40E)Bri 18 mouse these mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the coding region for SV40 T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 55 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of cell lines isolated from pancreatic tumours deposited at ECACC. Cells secrete somatostatin and insulin in culture. Adherent clumps of small cells.

Aplicação

Study of exocrine pancreas carcinomas, hormone secretion studies.

Meio de cultura

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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