HF1-5

94101909, rat liver, Epithelial

• Código UNSPSC: 41106514
• fonte biológica: rat liver
• modo de crescimento: Adherent
• cariótipo: 100 chromosomes
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• doença(s) relevante(s): cancer

Propriedades

• Nome do produto: HF1-5, 94101909
• fonte biológica: rat liver
• modo de crescimento: Adherent
• cariótipo: 100 chromosomes
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat hepatoma Hybrid [H5xFao]

Descrição de linhagem celular

HF1-5 is a subclone of HF1 selected for it′s ability to grow in glucose free medium. The entire group of hepatic functions are re-expressed. NB. The cells are difficult to cultivate, they do not like either low or high density and should be transferred frequently even though they grow slowly.

Aplicação

Gene analysis and liver function expression.

Meio de cultura

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split semi-confluent (60%-70%) cultures 1:5 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 37°C. Saturation density is reached at 5x1000,000 cells/10cm dish.

Outras notas

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