SK-PN-DW

94092301, human nerve, Polygonal

• Código UNSPSC: 41106514
• fonte biológica: human nerve
• modo de crescimento: Semi-adherent
• cariótipo: 82XXY
• morfologia: Polygonal
• produtos: Pro-cholecystokinin (Pro-cck)
• receptores: Vimentin, P53, anti-MIC2
• doença(s) relevante(s): cancer

Propriedades

• Nome do produto: SK-PN-DW, 94092301
• fonte biológica: human nerve
• modo de crescimento: Semi-adherent
• cariótipo: 82XXY
• morfologia: Polygonal
• produtos: Pro-cholecystokinin (Pro-cck)
• receptores: Vimentin, P53, anti-MIC2
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human primitive neuroectodermal tumour

Descrição de linhagem celular

SK-PN-DW has been derived from a male patient with a primitive neuroectodermal tumour that expresses cholecystokinin (cck) RNA and synthesises substantial amounts of pro-cck. SK-PN-DW is able to process the pro-hormone, producing immunoreactive cck-like material plus a peptide that immunochemically and chromatographically resembles intact cck c-terminal extension peptide. Cells have a tendency to float.

Aplicação

Tumour lines should prove useful in understanding the regulation of the cck gene in human neuronal cells and will also provide an in vitro system for investigation of the post translational processing of the cck preprohormone.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 9
D16S539: 12
D5S818: 13,14
D7S820: 8,14
THO1: 6
TPOX: 8,9
vWA: 16

Meio de cultura

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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