8305C

94090183, human thyroid, Epithelial

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: 8305C, 94090183
• fonte biológica: human thyroid
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human thyroid anaplastic carcinoma, undifferentiated.

Descrição de linhagem celular

Established from undifferentiated thyroid carcinomas of a 67 year old female patient. Pathologically the carcinoma tissue contained residual well differentiated components suggesting well differentiated to undifferentiated carcinoma progression. Tumour suppresser genes p53, Rb, APC and MCC were analysed and sequence analysis confirmed a C:G to T:A transition at the first base of p53 gene codon 273. Loss of heterozygosity of tumour suppresser genes was not observed.

Aplicação

As p53 mutations associate with undifferentiated carcinoma but not well differentiated carcinoma during multistep carcinogenesis of the thyroid, these cell lines should prove useful for research into the role of gene mutation in malignant transformation.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 9,12
D13S317: 9
D16S539: 10,11
D5S818: 10,13
D7S820: 8,10
THO1: 6,7
TPOX: 8
vWA: 14,16

Meio de cultura

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Doubling time is 43 hours. Saturation density at confluency is 5.7x10,000 cells/cm².

Outras notas

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