L6.C11

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92102119, rat muscle, Fibroblast

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: rat muscle
• embalagem: tube of 5 μg 92102119-DNA-5UG; pkg of vial of cells 92102119-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat skeletal muscle myoblast

Descrição de linhagem celular

A subclone of L6, a rat thigh muscle cell line. On reaching confluence the cells will fuse to form myotubes and twitching movement will be seen. Once cultures have fused they cannot be recloned.

Meio de cultura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split semi-confluent cultures to a seeding density of 1.5-2x1,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells fuse on reaching confluence, producing myotubes. To prevent fusion maintain the cultures sub-confluent and reclone every 6-8 weeks.

Outras notas

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