HT29 gluc C1

92012401, human colon, Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: HT29 gluc C1, 92012401
• fonte biológica: human colon
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: Elevated levels of cystic fibrosis transmembrane conductance regulator (CFTR) protein
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian colon adenocarcinoma

Descrição de linhagem celular

A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.

Meio de cultura

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO₃) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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