MKL-1

09111801, human skin. Loose floating aggregates with irregular outline and no central necrosis.

• Sinônimo(s): MKL1 Cells
• Código UNSPSC: 41106514

Propriedades

• Nome do produto: MKL-1, from human skin, 09111801
• fonte biológica: human skin
• modo de crescimento: Suspension
• cariótipo: Hyperdiploid
• morfologia: Loosely packed floating aggregates with irregular outline and no central necrosis
• produtos: MKL-1 is positive for CM2B4/MCV T AE1/AE2, CK20, EMA, CAM5.2, NSE and TTF-1. MKL-1 is negative for CK7, chromagen, synaptophysin, neurofilament, LCA, vimentin and CD99.
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): metastasis
• Condições de expedição: dry ice

Descrição

Origem de linhagem celular

Human neuroendocrine skin carcinoma

Descrição de linhagem celular

MKL-1 was established from a nodal metastasis in a 26-year-old Caucasian male patient. MKL-1 cells contain a Merkel cell polyomavirus integrated into the genome. MKL-1 cells cause tumour formation at 4-6 weeks at subcutaneous sites in athymic male nude mice (BALB/c nu/nu).

Perfil do DNA

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 10,12
D5S818: 11,12
D7S820: 8,11
THO1: 9,9.3
TPOX: 8
vWA: 16,18

Meio de cultura

RPMI 1640 + 2 mM Glutamine + 10% FBS

Rotina de subcultura

Seed at 3 x 10⁵ cells/ml in 5% CO₂; 37 °C Saturation density is approximately 10⁶ cells/ml. Population doubling time is around 120 hours.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.