GPNT

9061601, rat brain, Fusiform

• Código UNSPSC: 41106514
• fonte biológica: rat brain
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fusiform
• produtos: GPNT cells express tight junction proteins
• receptores: P-glycoprotein, PECAM-1, ICAM-1, VCAM-1 Glut-1, transferrin receptor, MHC class I

Propriedades

• Nome do produto: GPNT, from rat brain, 09061601
• fonte biológica: rat brain
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fusiform
• produtos: GPNT cells express tight junction proteins
• receptores: P-glycoprotein, PECAM-1, ICAM-1, VCAM-1 Glut-1, transferrin receptor, MHC class I
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice

Descrição

Origem de linhagem celular

An immortalised Lewis rat brain vascular endothelial cell line

Descrição de linhagem celular

GPNT was derived from the immortalised rat brain endothelial cell line GP8 by lipofectin-mediated transfection with a vector containing the puromycin resistance gene. GPNT exhibits a non-transformed phenotype.

Perfil do DNA

Not specified

Meio de cultura

Hams F10 + 10% Heat-inactivated FCS + 2 mM Glutamine + 2 ng/ml basic fibroblast growth factor + 80 μg/ml heparin. All plasticware is pre-coated for several hours with collagen (Type I from calf skin) at 10μg/cm2 from a 0.01% solution in acetic acid. After removal the coated plasticware is exposed to a mMonia vapour for 5 min. Plasticware must be rinsed thoroughly with PBS prior to use.

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 10hrs.

Outras notas

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Exoneração de responsabilidade

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.