328/14

8062541, mouse nerve (fibroblast), Neuronal/fibroblastic

• Código UNSPSC: 41106514
• fonte biológica: mouse nerve (fibroblast)
• modo de crescimento: Adherent
• cariótipo: Modal No. varied with passage see reference
• morfologia: Neuronal/fibroblastic
• produtos: Not specified
• receptores: Not specified
• doença(s) relevante(s): cancer

Propriedades

• Nome do produto: 328/14, 08062541
• fonte biológica: mouse nerve (fibroblast)
• modo de crescimento: Adherent
• cariótipo: Modal No. varied with passage see reference
• morfologia: Neuronal/fibroblastic
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse neuroblastoma x mouse L cell fibroblast hybrid

Descrição de linhagem celular

Hybrid generated by fusion of 6-thioguanine-resistant mutant of mouse neuroblastoma N4TG3 (Sigma Catalogue number 08062515) and 8-bromodeoxyuridine-resistant mutant of mouse L cell fibroblast line B82 (Sigma Catalogue number 08062522) using inactivated Sendai virus. The fusion of N4TG3 x B82 generated two types of hybrid cells: neuronal resembling the neuroblastoma and epithelioid with clearly visible nucleus. 328/14 is of the neuronal type.

Aplicação

Used as a neuronal Model

Meio de cultura

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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