N1E-115-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 8062511, mouse nerve, neuronal

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: mouse nerve
• embalagem: tube of 5 μg 08062511-DNA-5UG; pkg of vial of cells 08062511-1VL
• modo de crescimento: Adherent
• cariótipo: modal no. of chromosomes is 192 see reference
• morfologia: neuronal
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse neuroblastoma

Descrição de linhagem celular

A subclone of the cell line N1E-115 established by Amano et al. The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300. (Mouse strain A/Jax). N1E-115 cells are reported to be slightly contaminated with with a cell type of the morphology of C6 glioma cells. It is recommended to use N1E-115-1, a subclone of N1E-115, that is devoid of the contamination.

Aplicação

can be used as a neuronal model

Meio de cultura

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Population doubling approximately 20 hours.

Outras notas

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