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Documentos Principais

C7401

Sigma-Aldrich

Anti-Lectin, Canavalia ensiformis antibody produced in rabbit

fractionated antiserum, lyophilized powder

Sinônimo(s):

Anti-Con A, Anti-Jack Bean Concanavalin A

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

rabbit

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

fractionated antiserum

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

Formulário

lyophilized powder

reatividade de espécies

Canavalia ensiformis

embalagem

vial of ~2 mL lyophilized antiserum

técnica(s)

indirect ELISA: 1:25,000

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Imunogênio

Jack Bean Concanavalin A (Con A).

Aplicação

Anti-Lectin, Canavalia ensiformis antibody produced in rabbit has been used in:
  • enzyme-linked immunosorbent assays (ELISA)
  • immunochemistry
  • enhanced chemiluminescence ECL western blot analysis
  • western blot

Ações bioquímicas/fisiológicas

Concanavalin A is a lectin isolated from Canavalia ensiformis or Jack bean seeds. It has potent mitogenic effect on T cells and triggers the activity of both helper and suppressor T cells exhibiting immune regulator activity. Con A triggers autophagy by binding on the cell membrane glycoprotein and is internalized to the mitochondria. It activates immune response in liver and displays anti-hepatoma effect.

Descrição-alvo

Lectins are highly specific carbohydrate binding proteins useful in carbohydrate and cell membrane studies, as well as cell agglutination and typing techniques.

forma física

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2.

Reconstituição

Reconstitute with 2 mL deionized water.

Armazenamento e estabilidade

Prior to reconstitution store the product at 2-8 °C. After reconstitution, the solution may be stored frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage clarify the solution by centrifugation before use.

Informações legais

ECL is a trademark of Cytiva

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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B Fairburn et al.
Biochimie, 88(9), 1165-1174 (2006-05-13)
The stress protein gp96 exhibits a number of immunological activities, the majority of studies into which have used gp96 purified from a variety of tissues. On the basis of 1-D gel electrophoresis, the purity of these preparations has been reported
Nicolas Sauvion et al.
Journal of insect physiology, 50(12), 1137-1150 (2005-01-27)
Concanavalin A (lectin from Canavalia ensiformis L., ConA) has previously been shown to act as a feeding inhibitor for Acyrthosiphon pisum, the pea aphid. In the present study a range of histochemical and biochemical techniques were used to elucidate the
The use of concanavalin A to study the immunoregulation of human T cells.
J M Dwyer et al.
Clinical and experimental immunology, 46(2), 237-249 (1981-11-01)
M Malerba et al.
Biochemical and biophysical research communications, 286(5), 984-990 (2001-08-31)
Several authors previously showed that the interaction between 14-3-3 proteins and plasma membrane H(+)-ATPase leads to an activated complex in which the enzyme is endowed with more favorable kinetic parameters and a more physiological pH optimum. In this paper we
Hai-Xia Wang et al.
World journal of gastroenterology, 18(2), 119-125 (2012-01-19)
As a chronic inflammatory disease of the liver, the pathogenic mechanisms of autoimmune hepatitis (AIH) have not yet been elucidated, with prognosis and diagnosis remaining unsatisfied. Currently the only viable treatments of AIH are immunosuppressant application and liver transplantation. It

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