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Key Documents

C-39891

Sigma-Aldrich

Hematopoietic Progenitor Expansion Medium DXF

Cytokine Mix E for 500 ml

Sinônimo(s):

Primary Cell Culture Media

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.71

forma

aqueous solution

embalagem

pkg of 1 vial

técnica(s)

cell culture | mammalian: suitable

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Descrição geral

Cytokine Mix E for HPC-Expansion Medium DXF

Aplicação

Cytokine Mix E is a ready-to-use mixture of growth factors optionally available for the HPC Expansion Medium DXF (C-28021). The animal component-free, defined formula contains recombinant human TPO, SCF, flt-3 ligand and IL-3. The optimized formulation allows vigorous expansion of hematopoietic progenitor cells, while maintaining their primitive phenotype. Differentiation is largely inhibited.

Qualidade

All lots of PromoCell Hematopoietic Progenitor Media are subjected to comprehensive quality control tests using primary human mononuclear cells as well as purified hematopoietic progenitor cells from cord blood. Each lot of PromoCell HPC Expansion Medium DXF is tested for the ability to support the expansion of hematopoietic progenitor cells with a primitive phenotype. Each lot of PromoCell Hematopoietic Progenitor Medium is tested for the capacity to induce directed differentiation into hematopoietic lineages using purified CD34+ progenitor cells from cord blood. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Armazenamento e estabilidade

Store the Cytokine Mix at -20°C in the dark immediately after arrival. If stored properly, the it is stable until the expiry date stated on the label. Medium supplemented with Cytokine Mix E should be used within 2 weeks after addition of the cytokines. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Rotina de subcultura

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Artigos

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

Primary Human Mononuclear Cells and Growth Media

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

Protocolos

In Vitro Expansion of CD34+/CD38- Human Hematopoietic Progenitor Cells in a Serum-free and Xeno-free Stem Cell Culture Media

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

Human CD34+ progenitor cells and xeno-free, defined media for the expansion of hematopoietic progenitor cells. Protocols for handling, cell passaging, media, and product use.

Hematopoietic Stem Cell Culture Protocols

Step-by-step hematopoietic stem cell culture protocols for isolation, expansion and differentiation of CD34+ hematopoietic progenitor cells including CFU assays. It can be cultured under defined conditions designed either to promote self-renewal and increase the number of primitive cells.

Promocell

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

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