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Documentos Principais

C-39615

Sigma-Aldrich

Osteoblast Growth Medium

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

Sinônimo(s):

Recommended with C-27010

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.81

forma

aqueous solution

embalagem

pkg of 1 vial

técnica(s)

cell culture | mammalian: suitable

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Descrição geral

Osteoblast Growth Medium SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-27010

Aplicação

Osteoblast Growth Medium has been optimized for the in vitro cultivation of human osteoblasts. The fetal calf serum in the medium is strictly pretested for optimal performance. The medium is optimized for primary human cells, but can also be used for bovine, murine, and rat osteoblasts as well as osteoblast cell lines.Recommended for:Human Osteoblasts (HOB)

Componentes

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixSupplementMix: Contains all media supplements premixed in one vial

Qualidade

All lots of PromoCell Osteoblast Growth Medium are subjected to comprehensive quality control tests using primary human osteoblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested osteoblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Nota de preparo

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.1 ml / ml

Armazenamento e estabilidade

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Rotina de subcultura

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Artigos

Primary Human Osteoblasts and Osteoblast Growth/Differentiation Media

Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.

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