C-39415

Melanocyte Growth Medium

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

• Sinônimo(s): Recommended with C-24210
• Código UNSPSC: 12352207
• NACRES: NA.81

Propriedades

• forma: aqueous solution
• embalagem: pkg of 1 vial
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Melanocyte Growth Medium SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-24210

Aplicação

Melanocyte Growth Medium is a serum-free, PMA (Phorbol Myristate Acetate)-containing Medium and has been developed for the in vitro cultivation of juvenile melanocytes. Since PMA is a tumor promoting mitogen, it may interfere with some experimental approaches. In these cases, we recommend using the PMA-free Melanocyte Growth Medium M2 (C-24300). The Melanocyte Growth Medium is optimized for primary human cells, but can also be used for equine, porcine, murine, and rat melanocytes.Recommended for:Normal Human Epidermal Melanocytes (NHEM), juvenile foreskin

Componentes

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualidade

All lots of PromoCell Melanocyte Growth Medium are subjected to comprehensive quality control tests using primary human melanocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested melanocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Nota de preparo

Final supplement concentrations after addition to the basal medium:Bovine Pituitary Extract: 0.004 ml / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 0.5 µg / mlPhorbol Myristate Acetate: 10 ng / ml

Armazenamento e estabilidade

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Rotina de subcultura

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Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable