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C-28030

Sigma-Aldrich

Mononuclear Cell Medium

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.81

Formulário

liquid

embalagem

pkg of 500 ml

técnica(s)

cell culture | mammalian: suitable

Condições de expedição

wet ice

Descrição geral

Mononuclear Cell Medium (Ready-to-use)

Aplicação

Mononuclear Cell Medium is a very rich short-term maintenance medium, which provides utmost cell viability for Mononuclear Cells from umbilical cord blood, adult peripheral blood and other sources, e.g. bone marrow. It is suitable to maintain Mononuclear Cells for up to 48 hours before proceeding with your experiments.Recommended for:Human Mononuclear Cells from Cord Blood (hMNC-CB)Human Mononuclear Cells from Peripheral Blood (hMNC-PB)

Qualidade

All lots of PromoCell Mononuclear Cell Medium are subjected to comprehensive quality control tests using primary human mononuclear cells from cord blood and adult peripheral blood. Each lot of PromoCell Mononuclear Cell Medium is checked for unaltered shortterm maintenance of highly viable mononuclear cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Nota de preparo

The Medium consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete Medium.

Armazenamento e estabilidade

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C. Note: The SupplementMix is delivered thawed and can be frozen after arrival without losing any activity

Rotina de subcultura

Click here for more information.

Código de classe de armazenamento

10 - Combustible liquids


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Artigos

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

Protocolos

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products on Sigma.com.

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

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