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Key Documents

C-27411

Sigma-Aldrich

Preadipocyte Growth Medium

Basal Medium, 500 ml

Sinônimo(s):

Recommended with C-39425 or C-39427 (for growth medium) or C-39436 or C-39437 (for Differentiation Medium)

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.81

forma

aqueous solution

embalagem

pkg of 500 ml

técnica(s)

cell culture | mammalian: suitable

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Descrição geral

Preadipocyte Basal Medium (supplements sold separately)

To form a complete Preadipocyte Growth medium, it should be combined with the media supplements: C-39425 or C-39427. To form a complete Preadipocyte Differentiation Medium, it should be combined with the media supplements: C-39436 or C-39437.

Aplicação

Preadipocyte Growth Medium is a low-serum (5% V/V) medium developed for the undifferentiated expansion of human preadipocytes. The media are optimized for primary human cells, but can also be used for murine preadipocytes.Recommended for:Human White Preadipocytes (HWP)

Componentes

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualidade

All lots of PromoCell Preadipocyte/Adipocyte Media are subjected to comprehensive quality control tests using primary human white preadipocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested preadipocytes. In addition, the Preadipocyte Differentiation Medium/Adipocyte Nutrition Medium are tested for the capacity to induce the terminal differentiation of preadipocytes into mature adipocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Armazenamento e estabilidade

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

Rotina de subcultura

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Artigos

Primary Human Preadipocytes and Preadipocyte/Adipocyte Growth & Differentiation Media

Primary human white preadipocytes and culture media for the expansion of undifferentiated preadipocytes, differentiation to adipocytes, and cultivation of mature adipocytes. Protocols for handling, cell passaging, media, and product use.

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