C-12642

Human Small Airway Epithelial Cells (HSAEpC)

500,000 cryopreserved cells

• Sinônimo(s): HSAEpC cells
• Código UNSPSC: 41106514
• NACRES: NA.81

Propriedades

• fonte biológica: human (bronchiole)
• embalagem: pkg of 500,000 cells
• morfologia: (epithelial)
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): asthma
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição geral

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Human Small Airway Epithelial Cells (HSAEpC) serve as model cell culture systems for in vitro immune response studies. The 3D culture of HSAEpC mimics the in vivo cells effectively. The 3D cells are more viable, functional and efficient for immune response studies compared to the 2D cells.

Primary Human Small Airway Epithelial Cells (HSAEpC) are isolated from the distal portion of the human respiratory tract in the 1 mm bronchiole area and are provided in a cryopreserved format. They stain positive for cytokeratin.The distal respiratory tract mainly consists of pulmonary alveoli, which are spherical outcroppings of the respiratory bronchioles and are the primary sites of gas exchange with the blood. HSAEpC are qualified for functional studies to investigate disorders such as asthma or pulmonary inflammation.

Origem de linhagem celular

Bronchiole

Aplicação

Human Small Airway Epithelial Cells (HSAEpC) may be used:

• in overexpression of α_vβ₆ integrin
• in cytotoxicity studies with Cannabis sativa oil extract
• in transfection studies with highly pathogenic avian influenza virus and influenza A virus

Qualidade

Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Advertência

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rotina de subcultura

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Outras notas

Recommended Plating Density: 10000 - 15000 cells per cm²Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: > 15

Produtos recomendados

Recommended Primary Cell Culture Media:Link

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable