C-12208

Human Umbilical Vein Endothelial Cells 2 (HUVEC 2)

Pooled, isolated in Growth Medium 2, 500,000 cryopreserved cells

• Código UNSPSC: 12352207
• NACRES: NA.71

Propriedades

• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Aplicação

Primary Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from the vein of the umbilical cord. They are commonly used for physiological and pharmacological investigations, such as macromolecule transport, blood coagulation, angiogenesis, and fibrinolysis. HUVEC are isolated from pooled donors (from up to four different umbilical cords) in Endothelial Cell Growth Medium 2, which is free of ECGS and contains additional IGF and VEGF, and provided in a cryopreserved format. (Human Umbilical Artery Endothelial Cells (HUAEC) from the same donor are available on request.)

Qualidade

Rigid quality control tests are performed for each lot of Endothelial Cells from large vessels. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. von Willebrand Factor (vWF) and CD31, as well as Dil-Ac-LDL uptake assays are also carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Advertência

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rotina de subcultura

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Outras notas

Recommended Plating Density: 5000 - 10000 cells per cm²Passage After Thawing: P1Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Produtos recomendados

Recommended Primary Cell Culture Media:Link

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable