B3670

Betacellulin human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Sinônimo(s): BTC
• Número CAS: 163150-12-7
• Número MDL: MFCD00676521
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• product name: Betacellulin human, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)
• fonte biológica: human
• Nível de qualidade: 100
• recombinante: expressed in E. coli
• Ensaio: ≥97% (SDS-PAGE)
• forma: lyophilized powder
• potência: 0.1-0.6 ng/mL ED₅₀
• peso molecular: protein 9.5 kDa
• embalagem: pkg of 10 μg
• condição de armazenamento: avoid repeated freeze/thaw cycles
• técnica(s): cell culture | mammalian: suitable
• Impurezas: endotoxin, tested
• nº de adesão UniProt: P35070
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... BTC(685)

Descrição

Aplicação

This product has been used to study differentiation of pancreatic acinar cell line (AR42J) into insulin-secreting cells and has been linked to a role in the growth and differentiation of islet cells in human pancreas.

Ações bioquímicas/fisiológicas

Betacellulin, and the other members of the EGF family of growth factors, binds with high affinity to both EGFR and to erbB-4. Overexpression of Betacellulin with erbB-3 and erbB-4 has been linked to human endometrial adenocarcinoma.

Atenção

This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.

Nota de preparo

This product was lyophilized from a 0.2 μm filtered solution of PBS containing 5 mg of bovine serum albumin. It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 1 μg/mL.

Nota de análise

The biological activity of this product is measured by an ability to stimulate H-thmidine incorporation in EGF-responsive mouse BALB/c 3T3 fibroblasts. The EC₅₀ is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

Informações de segurança

• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)