B2787
Biotinylated Molecular Weight Marker
mol wt 6,500-180,000 Da
Sinônimo(s):
protein markers, protein molecular weight markers, protein standards
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About This Item
Código UNSPSC:
12352200
eCl@ss:
34058011
NACRES:
NA.25
Produtos recomendados
Formulário
lyophilized powder
Nível de qualidade
uso
1 vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)
peso molecular
6,500-180,000 Da
embalagem
vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)
temperatura de armazenamento
−20°C
Aplicação
For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins. Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli or the Weber and Osborn SDS-PAGE procedures.
Componentes
This product contains:
- α2-macroglobulin (equine serum)
- β-galactosidase (E. coli)
- phosphorylase b (rabbit muscle)
- catalase (bovine liver)
- alcohol dehydrogenase (yeast)
- carbonic anhydrase (bovine erythrocyte)
- trypsin inhibitor (soy bean)
- lysozyme (chicken egg)
- aprotinin (bovine lung)
Outras notas
Contains nine biotinylated proteins (approx 0.1 mg total).
Reconstituição
Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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D Della-Penna et al.
Analytical biochemistry, 152(2), 329-332 (1986-02-01)
Protein molecular weight standards were biotinylated by reaction with biotinyl-N-hydroxysuccinimide ester. The biotinylated proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose paper. The resolved protein bands were detected by formation of a streptavidin-biotin/horseradish peroxidase
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
K Weber et al.
The Journal of biological chemistry, 244(16), 4406-4412 (1969-08-25)
Eduardo L V Silveira et al.
Clinical and vaccine immunology : CVI, 15(8), 1292-1300 (2008-06-27)
Earlier studies have demonstrated in A/Sn mice highly susceptible to Chagas' disease protective immunity against lethal Trypanosoma cruzi infection elicited by vaccination with an open reading frame (ORF) expressed by amastigotes. In our experiments, we used this mouse model to
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
U K Laemmli
Nature, 227(5259), 680-685 (1970-08-15)
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