B0184

Bacteriorhodopsin from Halobacterium salinarum

native sequence, lyophilized powder

• Sinônimo(s): BR from H. salinarum, Bacterioopsin, Bacteriorhodopsin from Halobacterium halobium
• Número CAS: 53026-44-1
• Número MDL: MFCD00130599
• Código UNSPSC: 12352202
• NACRES: NA.56

Propriedades

• fonte biológica: Halobacterium salinarium
• Formulário: lyophilized powder
• técnica(s): ligand binding assay: suitable; mass spectrometry (MS): suitable
• nº de adesão UniProt: B0R5N9
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: Halobacterium salinarium ... OE_RS05715(5953595) , VNG_RS05715(144807)

Descrição

Descrição geral

Bacteriorhodopsin (BR) is a covalent complex comprising bacterioopsin protein and retinal cofactor in the equimolar ratio. It corresponds to the molecular weight of 27kDa. BR belongs to the retinylidene class of proteins. It is a seven-membrane helical protein that acts as a photon-driven pump. BR can be used in studies of the folding and kenetics of β-helical proteins.

Bacteriorhodopsin is the prototypical "seven-helix" transmembrane protein (with seven α-helical domains), whose study led to advances in understanding G protein-coupled receptors (GPCRs). In Halobacteria, it acts as a light-harvesting protein, producing a proton gradient across the cell wall that is then used to drive biosynthetic processes.

Aplicação

Bacteriorhodopsin from Halobacterium salinarum has been used:

• in generation of droplet lipid bilayer
• as a standard in quadrupole time-of-flight (QTOF) mass spectroscopy (MS)
• in the generation of protein-detergent complex and micelles for dynamic light scattering studies

Bacteriorhodopsin is of interest in the development of artificial retinas, optical associative processors, and three-dimensional memory storage devices.

Ações bioquímicas/fisiológicas

A transmembrane retinylidine protein that functions as a proton pump driven by light energy in Holobacterium.

Bacteriorhodopsin (BR) from Halobacterium salinarum acts as a proton-driven pump. BR can be used in studies of the folding and kinetics of α-helical proteins. It is thermally stable and exhibits high photoelectric and photochemical efficiency. BR exists as trimer in a hexagonal lattice. Its photocycle intermediates are exploited in bioelectronics majorly in photoelectric and photochemical applications.

Nota de preparo

Aqueous suspensions may be sonicated to achieve the desired homogeneity and may be stored for a short time at a temperature of 2-8 °C or at a temperature of -20 °C without time limitation.

Wild-type bacteriorhodopsin is isolated from Halobacterium salinarum strain S9 as purple membranes.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)