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Documentos Principais

A8062

Sigma-Aldrich

Anti-Goat/Sheep IgG−Alkaline Phosphatase antibody, Mouse monoclonal

clone GT-34, purified from hybridoma cell culture

Sinônimo(s):

Monoclonal Anti-Goat/Sheep IgG, Monoclonal Anti-Goat/Sheep IgG–Alkaline Phosphatase antibody produced in mouse

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

mouse

conjugado

alkaline phosphatase conjugate

forma do anticorpo

purified from hybridoma cell culture

tipo de produto de anticorpo

secondary antibodies

clone

GT-34, monoclonal

Formulário

buffered aqueous glycerol solution

reatividade de espécies

sheep, goat, bovine

não deve reagir com

rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human

técnica(s)

direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): 1:150,000 using total cell extract of chicken fibroblasts (10-20 μg per well)

Isotipo

IgG1

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

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Descrição geral

IgGs are glycoprotein antibodies that modulate several immune responses. Primary goat against target proteins are often used in various research applications. Thus, secondary anti-goat IgGs can be useful tools for the analysis of target proteins. However, secondary antibodies may lack species specificity as they may bind to non-related Igs in the test preparation. This may lead to increased levels of background staining. Hence, use of monoclonals can effectively reduce background staining and increase the specificity of immunoassays.
Monoclonal Anti-Goat/Sheep IgG-Alkaline Phosphatase recognizes goat and sheep IgGs. No cross-reactivity is observed with human IgGs or IgGs derived from the following species: guinea pig, rat, horse, dog, chicken, pig, mouse, rabbit, and cat.
The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.

Imunogênio

Purified goat IgG

Aplicação

Mouse monoclonal anti-goat/sheep IgG-alkaline phosphatase antibody has been used as a secondary antibody for ELISA assays at 1:4,000 and 1:15,210 dilutions. The product can also be used for IHC (1:40) and western blot applications (1:150,000).
The level of IL-5 in HPAEC conditioned medium was determined by ELISA using alkaline phosphatase-conjugated monoclonal mouse anti-goat/sheep IgG (GT-34) as the secondary antibody.

forma física

Solution in 0.05 M Tris buffer pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análise (COA)

Lot/Batch Number
109M4852V
21191003
037M4866V
21170237
035M4847V

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