A1287

5-Azacytidine

Hybri-Max^™, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

• Sinônimo(s): 4-Amino-1-(β-D-ribofuranosyl)-1,3,5-triazin-2(1H)-one, Ladakamycin
• Fórmula empírica (Notação de Hill): C₈H₁₂N₄O₅
• Número CAS: 320-67-2
• Peso molecular: 244.20
• Beilstein: 620461
• Número CE: 206-280-2
• Número MDL: MFCD00006539
• Código UNSPSC: 12352207
• ID de substância PubChem: 24890525
• NACRES: NA.75

Propriedades

• fonte biológica: synthetic (organic)
• Nível de qualidade: 200
• grau: Hybri-Max^™
• esterilidade: γ-irradiated
• linha de produto: BioXtra
• forma: lyophilized powder
• técnica(s): cell culture | hybridoma: suitable
• Impurezas: endotoxin, tested
• pf: 226-232 °C (dec.) (lit.)
• solubilidade: acetic acid: water (1:1): 5 mg/mL, clear, colorless
• espectro de atividade do antibiótico: viruses
• Modo de ação: DNA synthesis | interferes
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: NC1=NC(=O)N(C=N1)[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O
• InChI: 1S/C8H12N4O5/c9-7-10-2-12(8(16)11-7)6-5(15)4(14)3(1-13)17-6/h2-6,13-15H,1H2,(H2,9,11,16)/t3-,4-,5-,6-/m1/s1
• chave InChI: NMUSYJAQQFHJEW-KVTDHHQDSA-N
• Informações sobre genes: human ... DNMT1(1786) , DNMT3A(1788)

Descrição

Descrição geral

Chemical structure: nucleoside

Aplicação

5-Azacytidine may be used to induce in vitro cardiomyogenic differentiation of adult human bone marrow mesenchymal stem cells. It may also be used to study the effects of 5-Azacytidine on the immune regulatory myeloid-derived suppressor cells (MDSCs) and regulatory T cells (Tregs).

Used to study the effects of the DNA incorporated molecule on DNA methylation processes.

Ações bioquímicas/fisiológicas

5-Azacytidine is a deoxycytidine analog and a demethylating agent. It acts as a potential antineoplastic agent for acute myelogenous leukemia. 5-Azacytidine activates repressed genes by inhibiting DNA methylation. 5-Azacytidine also affects protein synthesis by altering the RNA function and stability.

A potent growth inhibitor and cytotoxic agent; inhibits DNA methyltransferase, an important regulatory mechanism of gene expression, gene activation and silencing.

Reconstituição

Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500ml medium. Final working concentration: 10 μM 5-azacytidine.

Informações legais

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Informações de segurança

• Pictogramas: GHS08,GHS07,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H302,H341,H350,H360FD,H372,H410
• Declarações de precaução: P202 - P260 - P264 - P273 - P301 + P312 - P308 + P313
• Classificações de perigo: Acute Tox. 4 Oral - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1B - STOT RE 1
• Código de classe de armazenamento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de risco de água (WGK): WGK 3
• Equipamento de proteção individual: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges